For imino acids, gelatin had proline and hydroxyproline of 127 and 99 residues/1,000 residues, respectively.
Amino acid compositions of gelatin from goat skin pretreated with 0.75 M NaOH for 2 days, compared with those of bovine and porcine gelatins Amino acids Residue/1,000 residues Goat Bovine Porcine gelatin gelatin * gelatin ** Aspartic acid/asparagine 45 44 54 Threonine 18 17 25 Serine 32 29 41 Glutamic acid/glutamine 73 74 81 Glycine 329 341 328 Alanine 110 115 114 Cysteine 1 0 0 Valine 20 21 18 Methionine 6 5 12 Isoleucine 11 11 9 Leucine 25 25 20 Tyrosine 3 1 2 Phenylalanine 13 12 14 Hydroxylysine 6 7 8 Lysine 28 26 22 Histidine 5 5 8 Arginine 49 48 45 Hydroxyproline 99 96 70 Proline 127 123 129 Total 1,000 1,000 1,000 Imino acids 226 219 199 * Jellouli et al.
In conclusion, the advantages of the PITC HPLC method, such as high resolution, speed, and sensitivity for quantifying of
imino acids, sulfur, and [beta]-amino acids, make it suitable for urine amino acid analysis, and its reliability and feasibility for clinical use has been demonstrated.