high-per·for·mance liq·uid chro·ma·tog·ra·phy (HPLC),

a chromatographic technology used to separate and quantitate mixtures of substances in solution. A sample is injected into a moving stream of solvent that flows through a column and detector. Separation during passage through the column occurs by absorption, partition, ion exchange, or size exclusion. The technique is commonly used in laboratories to measure organic compounds including steroid hormones, pesticides and poisons, toxic and carcinogenic compounds, and drugs.

Synonym(s): high-pressure liquid chromatography

Farlex Partner Medical Dictionary © Farlex 2012

high-per·for·mance liq·uid chro·ma·tog·ra·phy

(HPLC) (hī pĕr-fōr'măns lik'wid krō'mă-tog'ră-fē)

A chromatographic technology used to separate and quantitate mixtures of substances in solution. The technique is used to measure organic compounds, including steroid hormones, pesticides and poisons, toxic and carcinogenic compounds, and drugs.
Synonym(s): high-pressure liquid chromatography.

Medical Dictionary for the Health Professions and Nursing © Farlex 2012

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References in periodicals archive

Determination of total homocysteine in human plasma by isocratic high-performance liquid chromatography. Eur J Clin Chem Clin Biochem 1997; 35:687-91.

Critical role of pH for derivatization of homocysteine with benzofurazanes

Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-high pH buffer eluent.

Integration of olanzapine determinations in a HPLC-diode array detection system for routine psychotropic drug monitoring

Identification of fenthion and temephos and their transformation products in water by high-performance liquid chromatography with diode array detection and atmospheric pressure chemical ionization mass spectrometric detection.

Antiandrogenic activity and metabolism of the organophosphorus pesticide fenthion and related compounds. (Research)

Denaturing high-performance liquid chromatography detects reliably BRCA1 and BRCA2 mutations.

Novel mutation and polymorphisms of the HMBS gene detected by denaturing HPLC

With high-performance liquid chromatography and gas chromatography/mass spectrometry, Bada and student Meixun Zhao found that the Denmark section contained significant amounts of the two amino acids.

Rare amino acids support impact theory

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Update on undertaking extractable and leachable testing

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Peptide characterization and application protocols

High-performance liquid chromatography with multi-wavelength detection of the bisphenol-A impurities.

Bisphenol A and Dental Sealants: Olea's Response

Determination of [gamma]-glutamylglutathione and other low-molecular mass biological thiol compounds by isocratic high-performance liquid chromatography with fluorimetric detection.

Glutathione and glutathione metabolites in small tissue samples and mucosal biopsies

The researchers used a technique known as high-performance liquid chromatography to determine the length of phosphate chains in both the ion-damaged material and the glass.

Bombardment versus quick cooling