They focused on one part of
endoderm internalization: the hindgut, which gives rise to half of the small intestine, the large intestine, and colon.
After specification of the PGCs in the early mesendoderm ~2 weeks after conception, they make their way to the wall of the yolk sac
endoderm [45].
Differentiation of RUES2 cells to an intestinal lineage resulted in an efficiency of approximately 60% for both
endoderm and mid/hindgut differentiations (Figures 3(a) and 3(b), Table S2).
Mulato II, Llanero and Estrela-roxa presented a higher thickening of the walls of the
endoderm (Figure 1g), with an increase of intercellular spaces in the cortex of Mulato II.
(See figure) In placing the embryo outline over the iris chart, we see the areas of the ectoderm, the mesoderm, and the
endoderm relate to the location of the various organs and body structures.
The mouse embryo epiblast organizes initially from a ball of cells and becomes an epithelial layer in the form of a cup (cup-shaped) surrounded by visceral
endoderm (equivalent to the humans' hypoblast).
Background: The Definitive
Endoderm (DE) differentiation using the undefined media and non-human feeders can cause contaminations in the generated cells for therapeutic applications.
Only cells of the inner cell mass (ICM) from blastocyst have the pluripotent capacity for formation of all three primary germ layers,
endoderm, mesoderm, and ectoderm.
The first step of pancreatic development is primitive
endoderm (PrE) specification from pluripotent stem cells isolated from the mural surface of the inner cell mass of blastocysts (3-5 days post-fertilization in mice).