As a result, all endogenous amino compounds produce fluorescent derivatives, which produce very large solvent peaks, decreasing the sensitivity and increasing the length of the
chromatographic run times.
In order to realize the chemical composition change in
chromatographic peak of fingerprints, the sample from medicinal materials to pharmaceutical preparations using the same HPLC condition as fingerprint except mobile phase of 0.1% formic acid instead of 0.1% phosphoric acid by LC/QTOF-MS.
According to the International Union of Pure and Applied Chemistry (1), affinity chromatography is defined as a liquid
chromatographic technique that makes use of a "biological interaction" for the separation and analysis of specific analytes within a sample.
[12], liquid
chromatographic determination of levamisole in animal plasma by Baere S et al.
Due to the basic character of isoquinoline alkaloids, it would be preferable to conduct
chromatographic separation at alkaline pH to avoid their ionization; however, silica-based adsorbents are unstable at this condition [21].
Summary: A high performance liquid
chromatographic method was developed and validated to simultaneously quantify citalopram with piroxicam, celecoxib and diclofenac sodium.