Although ST-APSCs have the differentiation ability for three cell lineages, osteo-, chondro-, and adipogenic, originated from mesenchyme, they are less potent adult stem cells compared to DPSCs derived from wisdom teeth.
Differentiation medium contents used to differentiate stem cells to osteo-, chondro-, and adipogenic cell lineages Differentiation Content Osteogenic medium 100 nM dexamethasone, 10 mM [beta]-glycerophosphate, 0.2 mM ascorbic acid Chondrogenic medium 1x insulin--transferrin--selenium, 100 nM dexamethasone, 100 ng/ml TGF-[beta], 14 [micro]g/ml ascorbic acid, 1 mg/ml BSA Adipogenic medium 100 nM dexamethasone, 5 [micro]g/ml insulin, 0.5 mM 3-isobutyl-1-methylxanthine, 60 [micro]M indomethacin Table 2.
chondro- cytes produce cytokines.8 Either due to degenerative changes or disc injury, the herniated disc becomes vascularized.3
The study showed that through this approach, it promoted ADSC-mediated
chondro- and osteogenesis, and the PRP/insulin-induced differentiation was independent of IGF-1R signaling.
in a rabbit model [32], the time course of
chondro- and osteogenesis in an osteochondral defect is well known.
Previous reported data shows that hTERT-transduced mesenchymal stem cells have prolonged replicative capacity in vitro and keep the adipo-,
chondro-, and osteogenic differentiation potential in vitro and osteogenic potential in vivo [27, 28].
Following purification by FACS, MECs (CD34+/CD56+/CD144+/CD45-) can be clonally expanded and exhibited osteo-,
chondro-, adipo-, and myogenic differentiation capacities in vitro [25].