The immunogenicity of
chimeric antibodies. J Exp Med 1989:170(6):2153-2157.
Chimeric antibodies are composed of murine variable regions fused onto human constant regions.
Our observation was in agreement with the previous 2 reports of
chimeric antibodies with analogous specificity, albeit with different reactivity, vs those of the original antibody, because of immunoglobulin subclass (21, 22).
Antibodies to
chimeric antibodies (HACA) may be directed against a wide variety of epitopes in both the antigen combining site or framework epitopes in both the constant and variable regions of the immunoglobulin molecule.
[9] presented the treatment of a mouse model of
chimeric antibodies which blocked the intracellular PRL-3 protein.
In addition, functional single chain antibodies, antibody fragments and
chimeric antibodies may also be produced.
Wesolowski, "High-level expression of
chimeric antibodies using adapted cDNA variable region cassettes," Journal of Immunological Methods, vol.
The challenge of this method lies in being able to distinguish the tryptic peptides from the vast repertoire of endogenous immunoglobulins present in human serum, which is easier for
chimeric antibodies such as infliximab but can be more challenging for fully human antibodies such as adalimumab.