Based on the results obtained in experiment 1, this assay was conducted to evaluate exclusively the cholinergic agonists acetylcholine chloride,
carbamylcholine chloride (Nacalai Tesque Inc.), and succinylcholine chloride (Wako), and serotonin creatinine sulfate on the metamorphosis of a cohort of larvae of three age groups, 19, 21, and 23 days old.