capillary gel electrophoresis

A type of electrophoresis in which charged biomolecules (e.g., DNA) are separated by filling a capillary tube with a gel matrix, in which shorter strands of DNA migrate more quickly than longer strands. CGE can be used to identify single-base deletion products or truncated sequences from full-length nucleic acids by analysing inserted synthetic oligonucleotides.

References in periodicals archive

Although our method does not screen the entire dystrophin gene, it is rapid (all multiplex PCBs are amplified in ~2.5 h, and the capillary gel electrophoresis of the pooled PCR products takes ~30 min), easy to perform, detects ~99% patients with macrodeletions and 89% with macroduplications, and identifies small insertions or deletions.

Direct detection of exon deletions/duplications in female carriers of and male patients with Duchenne/ Becker muscular dystrophy

Screening and identification of familial defective apolipoprotein 13100 in clinical samples by capillary gel electrophoresis. J Chromatogr A 1996;744:187-194.

Capillary electrophoresis in the analysis of the deletion/insertion polymorphism of the angiotensin I-converting enzyme gene

Ultrasensitive near-IR fluorescence detection for capillary gel electrophoresis and DNA sequencing.

Rapid detection of 21-hydroxylase deficiency mutations by allele-specific in vitro amplification and capillary zone electrophoresis

Capillary gel electrophoresis (CGE) has been used for analysis of double-stranded DNA, including products of PCR [1-5].

Analysis of cyanine dye-labeled PCR product and restriction fragments by capillary electrophoresis and laser-induced fluorescence

Stability measurements of antisense oligonucleotides by capillary gel electrophoresis. J Chromatogr A 1995;709:181-95.

Diagnosis of Duchenne/Becker muscular dystrophy and quantitative identification of carrier status by use of entangled solution capillary electrophoresis

All content on this website, including dictionary, thesaurus, literature, geography, and other reference data is for informational purposes only. This information should not be considered complete, up to date, and is not intended to be used in place of a visit, consultation, or advice of a legal, medical, or any other professional.