Basidia 24.5-35 x 5.5-6.5 [mu]m, cylindro-clavate to clavate, tetrasporic, hyaline (Fig.
Basidia 18.0-20.2 x 5.8-6.8 [micro]m, clavate, 4-sterigmate, with basal clamp and oily contents; sterigmata up to 4.4 [micro]m long.
Basidia 16.0-28.0 x 5.6-6.8 [micro]m, clavate, 4-sterigmate, with basal clamp, with or without oily contents; sterigmata up to 4.8 [micro]m long.
Germination with phragmobasidia; often two-celled parts of the
basidia separate from the rest of the basidium, and plasma left in the teliospore germinates again; basidial cells develop basidiospores or hyphae.
Hymenium thickening;
basidia 23-28 X 6-7 [micro]m, clavate, clamped, yellowish and somewhat refringent under phase contrast; contents homogeneous or with minute granules or guttules, especially proximally; sterigmata 4, slender, more or less straight.
For morphoanatomical characterization of ectomycorrhizae, terminologies of Agerer (1991, 2006) were followed and sporocarps were analyzed macroscopically (color, lamellae, shape etc.) and microscopically (
basidia, basidiospores, cystidia etc.) following Reid (1984) methodology.
The measurements of spores,
basidia, cystidia and sterigmata were taken and drawings were made with the aid of a camera lucida.
Size ranges for 25 basidiospores, 20
basidia, 20 cheilocystidia were determined.
Size dimensions were determined for 25 basidiospores, 20
basidia, 20 cystidia, from each basidioma.A sample from a dried specimen of each species was ground in liquid nitrogen and placed in 2% CTAB buffer and DNA was extracted using Porebski et al.
Microscopic examinations (spore, hyphae,
basidia and cystidia) were performed using Leica DM LS2 microscope.