Digital
autoradiography allowed direct non-quantitative estimations of [sup.33]P activity uptakes between the treatments, being in the order of [sup.33]P-OrthoP >[sup.33]P-Fe [greater than or equal to] [sup.33]P-Al [greater than or equal to] [sup.33]P-NoP (Fig.
Left: representative digital
autoradiography of radioactive lipids of two different clones of CuFi-1-overexpressing GBA2 and in one clone of mock cells.
Precipitated proteins were then subjected to SDS-PAGE and
autoradiography to detect acylCoA incorporation (Figure 2(a)).
We concluded that COX staining and in situ hybridization of Syt7 and Syt4 mRNAs
autoradiography may be used as good markers for striatal and hippocampal injury induced by 3NPA.
The aim of the present study is to further investigate the alstonine mechanism of action by analyzing its effects on D2R binding in relevant brain areas (through quantitative
autoradiography) and dopamine uptake by striatal synaptosomes.
(b) TLC analysis (lanes 3, 4) and
autoradiography (lanes 1, 2) of the lipid fraction of both groups.
[37] Using whole-hemisphere
autoradiography (WHA), researchers found that compared to controls individuals with Type I alcohol dependence had a 20% reduction of D2/D3 receptor affinity in the NAc region and a 41% reduction in the amygdala.
Future studies employing
autoradiography are needed to confirm our results presented herein.
Using
autoradiography with the tritiated DNA nucleoside [sup.3]H-thymidine, Altman [27, 28] gained first evidence for the production of glia cells and possibly also of neurons in the brains of young adult rats and adult cats.