Sections of anterior, mid and posterior intestinal segments were also treated with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated.
To identify different contents of the goblet cells, the staining methods of alcian blue (AB) at pH 2.5 for acidic glycoconjugates with carboxylated and sulfate esters; aldehyde fuchsin (AF) for glycoconjugates with sulfate; periodic acid (0.5%)-Schiff reagent (PAS) for neutral glycoconjugates; potassium hydroxide/PAS (KOH/PAS) for glycoconjugates with sialic acid residues; and PAS/AB (pH 2.5) to distinguish between neutral and acidic glycoconjugates (Cinar and Senol, 2006) were performed (Presnell et al., 1997).