Agarose Gel Electrophoresis

A technique in which large biomolecules are separated on a highly purified agarose gel by electrophoresis. AGE is used in clinical chemistry to separate mixtures of proteins by charge and size, and in molecular biology to separate mixtures of nucleic acid (DNA and RNA) fragments by sieving (movement of molecules through the gel’s pores) and size, where shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through gel pores.

agarose gel electrophoresis

see GEL ELECTROPHORESIS.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

Second, the agarose gel electrophoresis requires that students analyze their gels carefully.

Analysis of the RNA content of the yeast Saccharomyces cerevisiae

Agarose gel electrophoresis of plasma DNA obtained from samples after HD revealed the typical apoptotic ladders.

Cell-free plasma DNA: a marker for apoptosis during hemodialysis

Evaluation of alkaline phosphatase isoenrymes in sera of hemodialysis and periton dialysis patients by agarose gel electrophoresis. Biochem Soc Trans 1995;23:310S.

Increased serum concentrations of intestinal alkaline phosphatase in peritoneal dialysis

Serum and urine agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE) were performed with, respectively, Paragon SPE Gel and Paragon Twin IFE Gel reagents (both from Beckman Coulter).

Heavy chain disease can be detected by capillary zone electrophoresis

The amplified PCR product was separated by agarose gel electrophoresis visualized by ethidium bromide staining and sequenced directly on an ABI PRISM 3100 Genetic Analyzer with ABI PRISM BigDye Terminator v3.0 sequencing kit (Applied Biosystems, Foster City, CA).

Coronaviridae and SARS-associated coronavirus strain HSR1

This relatively rapid clearance suggested a nonprotein compound, which was confirmed by the absence of an abnormal band on agarose gel electrophoresis (AGE; Paragon[R] SPE Kit; Beckman Coulter).

Pseudoparaproteinemia after lopamidol infusion for coronary angiography

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