With disease progression, the Smad7 levels were reduced with a concomitant increase in nuclear translocation of phosphorylated
Smad2, an indicator of active TGF-[beta]/Smad2 signaling.
T[beta]RI initiates phosphorylation of the adaptor proteins
SMAD2 and SMAD3 which is followed by the formation of complex with SMAD4 [5].
Mezey, "Transforming growth factor-fi1 up-regulation of human a!(I) collagen is mediated by Sp1 and
Smad2 transacting factors," DNA and Cell Biology, vol.
Immunoblots were probed with primary antibody against STAT3 (Cell Signaling Technology), pSTAT3 (Cell Signaling Technology), aSMA (Epitomics), E-cadherin (Bio-world), IL-22 (Millipore),
Smad2 (Cell Signaling Technology), pSmad2 (Cell Signaling Technology), or GADPH (Epitomics) at 4[degrees]C overnight followed by goat anti-rabbit secondary antibodies (Jackson ImmunoResearch, 1: 10,000 dilution) for 30 min at room temperature.
Lloyd, "Overexpression of
Smad2 drives house dust mite-mediated airway remodeling and airway hyperresponsiveness via activin and IL-25," American Journal of Respiratory and Critical Care Medicine, vol.
There are other genes Linked to body size that possibly could be associated with canine heart development: high mobility group AT-hook 2 (HMGA2), insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), Obscurin Like 1 (OBSL1), Stanniocalcin-2 (STC2), Suppressor Of Cytokine Signalling 2 (SOCS2) and Mothers Against Decantaplegic homolog (
SMAD2) (TGFb) (37), but this still is not clear.
The enriched TFs (Benjamini-Hochberg adjusted P value <0.2) of DEGs between Late and Early were
SMAD2, SMAD3, SPI1, STAT1, GATA2, and FLI1 [Figure 1]b.
Pathobiological mechanisms of peritoneal adhesions: The mesenchymal transition of rat peritoneal mesothelial cells induced by TGF-[beta]1 and IL-6 requires activation of Erk1/2 and
Smad2 linker region phosphorylation.