As can be seen in figures 4A and B in all fractions that were analyzed on SDS-PAGE, the overexpression band of the
recombinant protein was deposited in inclusion bodies and no expression band was observed in soluble fraction.
With the vast developments made in the transferring methods and transient culturing methods, the mass production of
recombinant proteins through transient transferring methods seems viable.
Concurrently,the company has begun an engineering project to convert the 9,000 square foot expansion space within CMF into cell culture and purification suites for production of
recombinant proteins expressed in mammalian cells.
In order to make the
recombinant protein expressed maximally, we compared the expression conditions as described in "Materials and Methods".
[1]
Recombinant Protein Laboratory, Biochemistry Department, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran;
ReFacto AF (moroctocog alfa)/XYNTHA (Antihemophilic Factor (Recombinant)) is a
recombinant protein drug for treatment and prevention of bleeding in patients with haemophilia A, a rare, chronic, genetic disorder in which the ability of a person's blood to clot is impaired due to missing or reduced levels of a protein known as factor VIII.
Recombinant protein was expressed in nine different media (LB ZB 4xZB ZBM ZYB 3xZYB M9 ZYBM9 and 3xZYBM9) by using Isopropyl AY-D-1-thiogalactopyranoside (IPTG) and lactose as inducer.
Expression of
recombinant protein in Escherichia coli (E.