IHC analysis of pocket proteins showed that 9-94% of the cells expressed the proliferation associated RB1 and 0-93% expressed the rest of phase protein RBL2. It is thus obvious that there are overlaps in expression of the RB1 and RBL2 proteins.
The Ki67 and cytometry analysis was complemented by an IHC based investigation of the pocket proteins RB1 and RBL2 (also known as P105 and P130).
Compared to the staining intensities for RBL2 and HP1[gamma], the signal was always found weaker in MLS tumor tissues and RB1 staining was also weaker in MLS tissues compared to reference tumor tissues of other entities.
(104-107) Recent genetic-expression profiling studies suggest that silencing of
RBL2, a tumor suppressor gene, in conjunction with MYC overexpression, may be important in the development of BL.
Recently, some miRNAs have been reported to regulate the limitless replicative potential of cancer cells such as the Dicer-dependent miR-290 cluster which can directly target the Retinoblastoma-like 2 protein (
Rbl2) and indirectly affects telomere integrity and telomere-length homeostasis (43).
(b) Gene symbol (c) Gene name (c) 2 PPM [O.sub.3] J02999 Rab2 ras-related protein RAB2 L19698 Rala GTP binding protein (Ral A) X07287 Pkrcg protein kinase C-[gamma] J03552 Mug1 plasma proteinase inhibitor D85760 Gna12 guanine nucleotide-binding protein a-12 M99567 PIcb3 phospholipase C [beta]-3 U00620 Cfs2 GM-CSF M59980 Kcnd2 voltage-gated K+ channel protein M83666 Hck Hck tyrosine protein kinase, p56 AF020777 Ptk2 focal adhesion kinase AF000300 Lyn lyn A tyrosine kinase 5 PPM [O.sub.3] U46034 Mmp11 matrix metal loproteinase 11 D55627
Rbl2 retinoblastoma-like 2 M95738 Slc6a11 Na+/K+ dependent GABA transporter M28647 Atp1a1 Na+/K+ATPaseed subunit U93306 Kdr VEGFR-2 M20637 Plcd1 phospholipase C delta 1 Accession no.