RAMP1

A gene on chromosome 2q36-q37.1 that encodes one of the RAMP (receptor [calcitonin] activity-modifying proteins) family of single-transmembrane-domain proteins, which are type-I transmembrane proteins needed to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on the expressed RAMP; in the presence of RAMP1, CRLR acts as a CGRP receptor. RAMP1 protein is involved in the terminal glycosylation, maturation and presentation of the CGRP receptor to the cell surface.

Mentioned in

CALCRL

References in periodicals archive

For quantitative analysis of the genes of interest [pre-pro-AM (Rn 00562327_m1), CRLR (Rn 00562334_m1), RAMP1 (Rn 01427056_m1), RAMP2 (Rn 00824652_m1), and RAMP3 (Rn 00571815_m1)], a commercially available TaqMan Assay-on-Demand System that consists of a kit of oligonucleotides and probes was used (Applied Biosystems).

The membranes were then incubated with the following specific antibodies (Santa Cruz Biotechnology, USA) overnight at 4[degrees]C: AM (sc-16496, 1:250 dilution), CRLR (sc-18007, 1:250), RAMP1 (sc-11379, 1:250), RAMP2 (sc-11380, 1:250), and RAMP3 (sc-11381, 1:250).

Figure 1A shows representative immunoblots for AM, CRLR, and RAMP1, -2, and -3 protein expression in rat CSM.

Pharmacological characterization of the relaxant effect induced by adrenomedullin in rat cavernosal smooth muscle

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