PYCARD

A gene on chromosome 16p11.2 that encodes an adaptor protein composed of two protein–protein interaction domains: an N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD), both of which belong to the six-helix bundle death domain-fold superfamily. This superfamily mediates assembly of large signalling complexes in the inflammatory and apoptotic-signalling pathways by activating caspase. In normal cells, PYCARD localises to the cytoplasm, but in cells undergoing apoptosis it forms ball-like aggregates near the nucleus.

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In the peritonitis mouse model (Table 2), PYCARD, IL-6, and lectin-like oxidized-LDL receptor-1 (LOX-1) were significantly upregulated in epididymal and in subcutaneous adipose tissue at all time points, whereas MCP-1 was induced only in epididymal but not in subcutaneous adipose tissue.

In the present study, we could demonstrate a shift in secretion profile from anti-inflammatory adipokines/cytokines such as adiponectin towards proinflammatory adipokines/cytokines such as visfatin, leptin, IL-6, PYCaRd, and MCP-1.

Innate Immunity of Adipose Tissue in Rodent Models of Local and Systemic Staphylococcus aureus Infection

The following primary antibodies were used: WNV (Envelope protein; Abcam, Cambridge, MA), Iba-1 (Wako Chemicals, Richmond, VA), CD3 and Mac-3 (BD Biosciences, San Jose, CA), Fas (X-20, Santa Cruz Biotechnology, Dallas, TX), caspase 3 (Asp175, clone 5A1E, Cell Signaling, Danvers, MA), and PYCARD ASC-speck (MyBiosource, San Diego, CA).

Transcription of other inflammasome components, the NLR family pyrin domain containing 3 (NLRP3) and PYD and CARD domain containing (PYCARD) proteins, was significantly upregulated in OPN KO animals, on day 5 pi compared to baseline (p values = 0.02 and 0.024, resp.) (Figures 5(b) and 5(d)).

Osteopontin Impacts West Nile virus Pathogenesis and Resistance by Regulating Inflammasome Components and Cell Death in the Central Nervous System at Early Time Points

The inflammasome is a multiprotein oligomer consisting of caspase-1, PYCARD, and NALP that mediate the response to cellular danger signals activating and recruiting inflammatory cells.

The effect of inflammatory cytokines in alcoholic liver disease