puromycin

(pyo͝or′ə-mī′sĭn)

An antibiotic, C₂₂H₂₉N₇O₅, obtained from the soil bacterium Streptomyces alboniger, that is used experimentally as an inhibitor of protein synthesis.

puromycin

Puromycin aminonucleoside Experimental biology An antibiotic that inhibits protein synthesis isolated from Streptomyces alboniger, used to induce experimental lipoid nephrosis in animals

References in periodicals archive

A, Screening of monoclonal cells by puromycin amino nucleoside.

Hypoxia-induced hyperpermeability of rat glomerular endothelial cells involves HIF-2[alpha] mediated changes in the expression of occludin and ZO-1

(c) Transduced hAD-MSCs after puromycin selection; abundant green cells and GFP expression exhibit high transduction level (d) FACS analysis of the transduction rate after puromycin selection indicated that more than 90% of the cells were GFP positive.

The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells

From above, cells were co-transfected with circular TALENs and linearized pBLC-TK, plated on 6-well plates and maintained at 37[degrees]C, under puromycin selection.

Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

Because the lentivirus vector contains both GFP gene and puromycin resistance gene, we used both markers for evaluation and selection of transductants.

Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro

Then, puromycin selection was performed in the presence of 2 mg/ml puromycin for 2 weeks.

Potent effects of dioscin against gastric cancer in vitro and in vivo

Infected cells were selected and continously maintained in the presence of 1.5 [micro]g/mL puromycin.

Different ATM signaling in response to chromium(VI) metabolism via ascorbate and nonascorbate reduction: implications for in vitro models and toxicogenomics

After 48 h, Hygromycin B (500 [micro]g/mL; Sigma-Aldrich), Neomycin (750 [micro]g/mL; Life Technologies), and Puromycin (5 [micro]g/mL; Sigma-Aldrich) were added to cell cultures.

Proteasome activators, PA28[alpha] and PA28[beta], govern development of microvascular injury in diabetic nephropathy and retinopathy

The [hUDSCs.sup.miR-375+] were exposed to 2.5 [micro]g/mL puromycin for 3days to obtain stable transduction.

Differentiation of umbilical cord mesenchymal stem cell toward insulin producing cells

After lentivirus infection and puromycin selection, the drug-resistant cells were obtained.

Stable Expression of T7 RNA Polymerase Mediated by Lentivirus in MDBK Cells