Following the morphological studies of bacterial colonies and cells, the biochemical properties of unknown bacteria were explored through their growth and colony color on two selective and differential media, MacConkey agar and mannitol salt agar, and the results of the
oxidase test. MacConkey agar contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria.
Further, characterization of the bacterial strain was performed based on
oxidase test, hanging drop technique, mannitol fermentation test, nitrate reduction test, TSI test and methyl red test (Qazilbash, 2002).
perfringens type D was confirmed through different biochemical tests (IMViC, Sugar fermentation, Catalase reaction,
Oxidase tests, Gelatin hydrolysis, Litmus milk reaction and Lecithinase) and PCR.
gram staining, motility, capsule, IMViC, cataalse,
oxidase tests as per methods described by Barrow and Felthem (1993).
GPC were identified by catalase, coagulase, and modified
oxidase tests. Gram negative bacilli (GNB) were identified by Motility, Oxidase, Catalase, IMViC, Urease, Triple Sugar Iron agar, Nitrate reduction.