The researchers compared the sensitivity and accuracy of
molecular beacon and TaqMan[R] probe PCR assays with the conventional USDA microbiological procedure using artificially contaminated RTE meats.
Since the
molecular beacon probe has the advantages of strong specificity, short hybridization time, and easy operation [19-22], it is expected to be applied in the miRNA rapid detection and analysis.
Ling, "Fluorometric determination of Simian virus 40 based on strand displacement amplification and triplex DNA using a
molecular beacon probe with a guanine-rich fragment of the stem region," Microchimica Acta, vol.
Currently, the World Health Organisation (WHO) has endorsed
molecular beacon technology for the direct detection of MTB by using ultra-sensitive polymerase chain reaction (PCR) technology.
This decision is a major independent validation of Miacom's proprietary
molecular beacon technology being successfully applied in the hemoFISH assays.
Detection is by a fluorescently-labeled
molecular beacon. Fluorescence is released as the fluorophore and quencher are separated when the beacon stem is opened.
For the signal system, TaqMan probe was used instead of
molecular beacon (Table 1).
The ANSR system uses an innovative isothermal DNA amplification process and fluorescent
molecular beacon technology for detection of the pathogen target.
MOLECULAR BEACON. Steven Xu Wu *, Julia Xiaojun Zhao
Rapid detection and quantitation of bluetongue virus (BTV) using a
molecular beacon fluorescent probe assay.
The sensitivity and accuracy of
molecular beacon and TaqMan[R] probe PCR assays were compared with the conventional USDA microbiological procedure using artificially contaminated RTE meats.