Similarly, galectin-1 (Lgals1) appeared to be represented by three clones; karyopherin [beta]1 (Kpnb1) and H3 histone 3B (H3f3b) were represented by two clones each.
The microarray analysis appeared to underestimate the fold change of expression compared with qPCR (Figure 3), with the only exception being Kpnb1, for which a downregulation in embryos was not supported by qPCR (Figure 3).
Although Kpnb1 is a gene whose expression has been reported to be downregulated by HDAC inhibition (Marks et al.