Although treatment with 3-MC alone had an effect on levels of Top2a, Ccnbl, and
Kif2c mRNA that was far less pronounced than for treatment with E2 alone, 3-MC significantly attenuated the up-regulation of Top2a, Ccnb2, and Mki67 by E2.
Gene name Fold change p value Process Gas2 12.27 0.001577852 Cytokinesis Tgfa 2.4 0.000103799 Growth factor Rapgef5 2.31 0.004103808 Ras activator Nuf2 2.24 0.002117132 Centromere Chl1 2.17 0.023016307 G2/M transition Top2a 2.04 0.002104745 DNA replication Cks2 1.92 0.007478331 CDK regulation Gins4 1.87 0.003569736 DNA replication Aspm 1.83 0.004862977 Spindle formation Cdk5r1 1.78 0.00289215 CDK regulation Prc1 1.75 0.005448853 Cytokinesis
Kif2c 1.75 0.000350709 Centromere Dscc1 1.73 0.008438809 DNA replication Kif15 1.66 0.008896449 Microtubules Ncapd2 1.65 0.004007301 DNA condensation Prim1 1.58 0.001821116 DNA replication
In the recent reclassification of breast cancers on the basis of copy number expression analysis, the 5q loss is associated with a strong basal-specific signature, including aurora kinase B (AURKB), B-cell CLL/lymphoma 2 (BCL2), BUB1 mitotic checkpoint serine/threonine kinase (BUB1), cell division cycle associated 3 (CDCA3), cell division cycle associated 4 (CDCA4), cell division cycle 20 (CDC20), cell division cycle 45 (CDC45), checkpoint kinase 1 (CHEK1), forkhead box M1 (FOXM1), histone deacetylase 2 (HDAC2), insulin-like growth factor 1 receptor (IGF1R), kinesin family member 2C (
KIF2C), kinesin family member C1 (KIFC1), methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), TTK protein kinase (TTK), and ubiquitin-conjugating enzyme E2C (UBE2C).
Cancer related (15 genes--43%) Hoxb13, Hmg20b, Cxcl16, Uimc1, Elf1, Foxp1, Ptgdr, Psmd8, Peg3, Myo15, Lig1, Ptbp2,
Kif2c, Grhpr#, Fah# Enzymes (5 genes--14%) Dck, Zdhhc4, Pik3c2g, Grhpr#, Fah# Diverse functions (7 genes--20%) Adipogenesis Aamdc Nuclear Rny1, Polr1d, Nol12, Notch signaling Dtx2 Vascular remodeling Reck/St15 Ig related S72845 Unknown function (10 genes--29%) AK010802, AK007003, AK016406, AK021335, AK015506, AK009275, AK007343, AK006390, AK006570, AK005580 Bolded gene names correspond to genes that fits into two groups (because are cancer related and also enzymes).