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Immunofluorescent assay

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Immunofluorescent assay (IFA)

A blood test sometimes used to confirm ELISA results instead of using the Western blotting. In an IFA test, HIV antigen is mixed with a fluorescent compound and then with a sample of the patient's blood. If HIV antibody is present, the mixture will fluoresce when examined under ultraviolet light.
Mentioned in: AIDS Tests
Gale Encyclopedia of Medicine. Copyright 2008 The Gale Group, Inc. All rights reserved.
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References in periodicals archive
Indirect immunofluorescent assay (IIFA) on individual specimens of Ae.
Detection of dengue viral infections in Aedes mosquitoes: an essential tool for epidemiological surveillance
Antibodies to phases I and II are measured using an immunofluorescent assay technique.
Laboratory outbreak of Q fever
We performed the sandwich 2-step immunofluorescent assay (IFA) as described (18).
Human pro-B-type natriuretic peptide is processed in the circulation in a rat model
Initial testing should use an enzyme immunoassay (EIA) or immunofluorescent assay (IFA); specimens yielding positive or equivocal results should be tested further by using a standardized Western immunoblot assay.
Caution regarding testing for Lyme disease
Serologic confirmation involves a two-test approach with a sensitive enzyme immunoassay or immunofluorescent assay and a Western immunoblot for IgG and 1gM antibodies.
Overdiagnosis and overtreatment of Lyme disease a problem on Long Island. (Only 32% Correctly Diagnosed)
Anti-HHV-8 antibodies were sought using a standardized indirect immunofluorescent assay, according to the manufacturer's protocol (HHV-8 immunoglobulin G [IgG] indirect immunofluorescent assay kit, Biotrin International Ltd, Dublin, Ireland).
Association of Primary Pleural Effusion Lymphoma of T-Cell Origin and Human Herpesvirus 8 in a Human Immunodeficiency Virus-Seronegative Man
The standard method employs either an enzyme-linked immunosorbent assay (ELISA) or an immunofluorescent assay (IFA) followed by a Western blot in positive or equivocal specimens.
Experts Differ on Chronic Lyme Disease Dx
In addition, serum samples were tested by indirect immunofluorescent assay for specific IgG and IgM against Francisella tularensis and Rickettsia spp.
African tick-bite fever in traveler returning to Slovenia from Uganda
Serological tests, such as indirect immunofluorescent assay (IFA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR), have produced satisfactory results.
Epidemiological and clinical characteristics of cutaneous leishmaniasis and their relationship with the laboratory data, south of Brazil
We performed affinity extraction as described (8), as was sandwich 2-step immunofluorescent assay (IFA) (8).
Processing of pro-b-type natriuretic peptide: furin and corin as candidate convertases
The patient was kept comatose for 7 days; during that period, results from lumbar puncture indicated an increase in antirabies IgG by immunofluorescent assay from 1:32 to 1:2,048.
Recovery of a patient from clinical rabies--Wisconsin, 2004
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