For the
HI test, we treated the samples with receptor-destroying enzyme (RDEII; Denka Seiken, http://www.keyscientific.com) at 37[degrees]C for 16 h, followed by heat inactivation at 56[degrees]C for 30 min.
(11) The test was considered positive when the HA end-point titre was depressed by three or more dilutions in the
HI test. A titre of 1/16 was considered positive.
Statistical analysis: The data regarding
HI test was processed for calculating geometric mean titer (GMT) on 21, 28 and 35 days post-vaccination (DPV).
Map of Harris County and the City of Houston, Texas, showing the geographical distribution of
HI test results for WNV, SLE, EEE, and WEE in 2015.
The virus Neutralization test (NT) was far more sensitive in comparison to
HI test (Orenstein et.
Antibody titer for NDV was determined from each serum sample using the OIE
HI test protocol.
The positive results showed that 81.7 % of ELISA positive cases had titer [greater than or equal to] 32 for H9N2 influenza in
HI test. Although positive result were found more in dogs with general or respiratory signs, no significant differences were observed in the evaluated factors and seropositivity.
The HA agent was confirmed as AI (H9 type) by using
HI test with AIV-H9 specific antisera (Muhammad et al., 2001).
Virus titer calculated by
HI test ranged from 1:64 to 1:512.
As a companion to the Hydraulic Institute Standards, together with the
HI Test Standards, the Engineering Data Book completes the data for solving problems related to the pumping and transfer of fluids.