MASPs additionallyform active complexes with Ficolin-1 (M-Ficolin), Ficolin-2 (L-Ficolin), and Ficolin-3 (H-Ficolin), which are also defence collagens [35-37].
Fujita, "Activation of the lectin complement pathway by H-ficolin (Hakata antigen)," The Journal of Immunology, vol.
Three members of the ficolin family of proteins have been described in humans, such as L-ficolin (or ficolin-2), H-ficolin (or ficolin-3 or Hakata antigen), and M-ficolin (or ficolin-1) [16-19].
H-ficolin is a protein of 34-kDa and thought to form oligomers of different sizes [3, 27, 32, 33].
cruzi, MBL as L- and H-ficolin is bound to N- and O-glycosylated molecules on the surface of T.
Aebi et al., "Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)," Pediatric Allergy and Immunology, vol.
Jensenius, "Congenital H-ficolin deficiency in premature infants with severe necrotising enterocolitis," Gut, vol.
We used sandwich ELISA methods to measure H-ficolin, L-ficolin, and mannose-binding lectin (MBL) concentrations in plasma samples.
After blocking the endogenous peroxidase with 30 g/L hydrogen peroxide, for ficolin staining alone, sections were incubated in TBS with normal sheep serum, with either H-ficolin monoclonal antibody (clone 4H5) from HyCult Biotechnology or rabbit polyclonal anti-L-ficolin antibody (clone 2F5) from Tokai University.
By tryptic peptide fingerprinting analysis, we confirmed that the identities of the 2 peptides were Hakata antigen and hucolin, known as H-ficolin and L-ficolin, respectively (Fig.
H-ficolin, L-ficolin, and MBLs were found to be maintained at very low concentrations (-3-5 mg/L) in healthy adults (8), but there is no previous report concerning lectin activity in normal pregnancies or pregnancies complicated by preeclampsia.
We confirmed that maternal plasma concentrations of H-ficolin and L-ficolin were significantly lower in preeclamptic pregnancies (n = 20) than in uncomplicated pregnancies (Fig.