Three most common methods, available in the literature, for determining the parameters of Michaelis-Menten equation based on a series of measurements of velocity v as a function of substrate concentration, are Lineweaver-Burk plot, also known as the double reciprocal plot, Eadie-Hofstee plot, and Hanes-Woolf plot.
Similarly, the Eadie-Hofstee plot has the disadvantage that v appears on both axes; thus, any experimental error will also be present in both axes.
Up to this day, these parameters are routinely estimated using one of these different linearization models: Lineweaver-Burke plot (1/ v versus 1/s), Eadie-Hofstee plot (v versus v/s), and Hanes-Wolfe plot (s/v versus v).
At least two components are involved in phenylalanine uptake in Y294[DELTA]agp1[DELTA]gap1 strain, as shown by the Eadie-Hofstee plot. The kinetic analysis indicates the presence of a nonsaturable component and one transport system of high-affinity with [K.sub.T] of 39 [micro]M and [J.sub.max] of 0.60 [micro]mol/min per g.
The Eadie-Hofstee plots indicate that phenylalanine uptake is mediated by a component of high affinity, presumably Gap1p.
For the strains Y294 and Y294[DELTA]bap2, the Eadie-Hofstee plots are clearly biphasic, whereas for the strain Y294[DELTA]agp1 the plot is linear.
However, a double reciprocal (Lineweaver-Burke) plot of these data resulted in nonlinear regresssion lines, which was confirmed by plotting the data in an Eadie-Hofstee plot. The observed nonlinear inhibition kinetics obscure the actual type of inhibition.
Inhibition kinetics were retrieved from double reciprocal (Lineweaver-Burke) and Eadie-Hofstee plots.
However, Lineweaver-Burke and Eadie-Hofstee plots revealed that the analytic variation in the data from the treatments with higher inhibitor concentrations was too high to draw reliable conclusions about the type of inhibition.