An innovative character of the
dot blot, which was used in the present study, is in the fact that in the SSHL diagnostics, in addition to free Hsp70, the Hsp70 bound to circulating immune complexes (CIC) is also searched for.
This result confirms previous studies [20,21] that used a
dot blot or indirect ELISA techniques based on extraction of rough soluble antigen from [RB.sub.51] vaccinal strain.
In the present study, we studied the distribution patterns of 5hmC, 5fC, and 5caC in the postnatal mouse brain, cultured adult neural stem cells (aNSCs) with immunostaining and
dot blot, and compared their global levels.
HAR is a common high-altitude illness, which is clinically characterised by increased dilatation and tortuosity of retinal vasculature along with diffuse
dot blot and flame shaped haemorrhages, usually involving mid-periphery.
The homogenate was centrifuged at 12,000 g for 20 min at 4 C and the resulting supernatant was used for
dot blot analysis.
The synaptosomes were further treated to isolate dendritic rafts and PSDs, and dendritic rafts and PSDs were analyzed by Western blot or
dot blot.
Protein Isolation, Western Blot, and
Dot Blot Analysis.
Furthermore, detection of TK1 protein was carried out in 96 patients using a chemiluminescence
dot blot assay.
The system can detect up to 22 targets per sample using reverse
dot blot technology and can detect genus, species, strains and virulence factors in a single assay.