Following incubation, all the isolates were sub-cultured onto
Czapek yeast agar (CYA), malt extract agar (MEA) and glucose-peptone-yeast extract agar (GPY), pH 3.7 to 4.5.
The fungi were isolated and grown in
Czapek Yeast Extract Agar (CYA) and Malt Extract Agar (MEA) for identification.
The spreading was performed using CYA medium (
Czapek yeast extract agar) containing (g [L.sup.-1]): [K.sub.2]HP[O.sub.4] (1), concentrated
czapek (10), agar (15), yeast extract (5), sucrose (30), and in MEA containing (g [L.sup.-1]): malt extract (20), bacteriological peptone (1), glucose (20), agar (20) supplemented with 10 mg of ZnS[O.sub.4]x7[H.sub.2]O and 5 mg of CuS[O.sub.4]x5[H.sub.2]O.
Sintesis de Acido indolacetico (AIA): Las bacterias se inocularon por triplicado en tubos viales de 22 ml conteniendo 15 ml del medio de cultivo
Czapek suplementando con 200 [micron]g/ml de DL-triptofano (SIGMA) y posteriormente se incubaron en oscuridad a 28[grados]C, en agitacion constante en Maxi-Shake (SBD PL 50 BIO) a 150 rpm durante 72 hr.
To produce secondary metabolites, the species were grown in
Czapek yeast broth.
Both traditional maisons and niche independent companies are participating in Salon des Grandes Complications, such as Armand Nicolet, Armin Strom, Blancpain, Breguet, Breitling,
Czapek, Faberge, Franck Muller, Girard-Perregaux, Greubel Forsey, Harry Winston, Hysek, Jaeger-LeCoultre, Montblanc, Panerai, Quinting, Roger Dubuis, Vacheron Constantin and Zenith, with retailers Alfardan Jewellery, Abdullah Said Binzagr Jewellery & Watches, Paris Gallery and Platinum Sands.
Table 1: Alteration of strength characteristics of concrete during 28 days of exposure in
Czapek Dox medium without inoculation (CDM) and inoculated by spores of Aspergillus fumigatus A2 and Penicillium brevicompactum P4.
Amended
Czapek's-agar medium containing 40 g lyophilized ground calf lung tissue as a sole carbon source was used.
Culture media of
Czapek agar and potato dextrose broth were procured from Lab M (Bury, Lancashire, UK).