In the cell cycle signaling pathway, we noted alteration in the expression
Ccna2 Stag2, Atr, Smc1a and Rbl1 .
For example, the let-7 family has been shown to repress multiple genes involved in cell cycle, cell apoptosis, and cell proliferation, including
CCNA2, CDC34, AURA/STK6, AURKB/ STK12, E2F5, and CDK8 [39].
Our data showed that several cell cycle related genes, such as DDIT3, PPP1R15A, and GADD45A, were upregulated in the propofol-treated group, while other genes that are involved in cell cycle progression, including
CCNA2, CDKN3, CDC2, and CDC25B, were downregulated.
Genes included in cluster 4 and 13 were related to cell cycle and renewal of stem cells (CCNB1,
CCNA2, FGF7, MAP2K4, WNT5A, KITGL, and FGF5).
The relative expression of CDK1, CDK2, CCNB1, GTSE1, ITPR1, c-MYC, SRGAP3, HIST2H3D, HIST] H3J, TOP2A,
CCNA2 and RHOJ was determined.
Aggressive follicular lymphoma, a subset of NHL, is associated with upregulation of genes involved in cell cycle control such as CCNE2 (cyclin E2),
CCNA2 (cyclin A2), CDK2 (cyclin-dependent kinase 2), and genes-reflecting increased metabolism and DNA synthesis [115].
Other upregulated genes were related to cell proliferation and its control, such as cyclin-dependent kinase gene CDK1/CDC2; cyclin genes
CCNA2, CCNB1, CCNB2, and CCNL2; the DLG7 component of the mitotic apparatus; the gene encoding the checkpoint kinase involved in response to DNA damage, CHEK1/CHK1; and BUB1 and MAD2L1, components of the spindle checkpoint.
Using miRGen and MicroCosm, we found that
Ccna2 and Bcl10, two genes that were up-regulated in our mRNA arrays, were the predicted targets of the down-regulated rno-miR-489 and rno-let-7e*, respectively.