PSK precursor polypeptides share a conserved domain close to the
C-terminus, from which mature functional PSK peptides are generated (Fig.
Moreover, 5-[HT.sub.2B] and 5-[HT.sub.6] receptors contain potential palmitoylation sites within their
C-terminus, although palmitoylation of these receptors was not demonstrated experimentally.
In HEK293T and A549 cells, PB1-F2 colocalizes and binds mitochondrial antiviral signaling protein (MAVS) via its
C-terminus and thereby inhibits MAVS-mediated type I interferon synthesis and decreases mitochondrial membrane potential [28, 29].
After optimization, a fusion protein was generated in which the N-terminus of dCas9 was linked to a nuclear localization signal (NLS), and the
C-terminus of dCas9 was linked to CIB1 with a truncated
C-terminus.
Since the
C-terminus of NuMA1 containing the nuclear localization signals (NLSs), we deduced that products encoded by the short isoform would mainly exist in the cytoplasm region, instead of the nuclear region or spindle poles.
All cholesterol binding toxins (CBT's) shows similarity in their primary amino acid sequence and identity in the so called undecapeptide region having tryptophan-rich loop that contains 11 amino acids, which is nearest to the
C-terminus of the polypeptide chain [17].
This SS cDNA encodes a protein with 114 amino acids that contains the SS14 sequence at its
C-terminus. This putative peptide is identical to that generated by the SS1 gene in other vertebrates.
Semitryptic peptides are peptides of which 1 of the 2 termini (in these cases: the
C-terminus) is not formed by trypsin cleavage, and can be used to identify proteolytic cleavage sites by proteases other than trypsin.
To confirm the translation of truncated MYOG protein from these mutated sequences, myogenic cells differentiated from Dox-treated hiPS cells for 7 days were immunoreacted with antibodies against human MYOG N-terminus and
C-terminus relatively because MYOG mRNAs are transcribed with the extra stop codon, which results from the MYOG gene targeting.