bacterial artificial chromosome

n. Abbr. BAC

A large DNA sequence that is artificially inserted into a bacterial genome for replication or expression.

bacterial artificial chromosome (BAC)

CLONING VECTOR based on the F PLASMID of ESCHERICHIA COLI, with the capacity to accept DNA inserts generally 100–300kb in size. Often used in the CLONE CONTIG SEQUENCING METHOD to obtain sequence CONTIGS in a genome sequencing project. See HUMAN GENOME PROJECT.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

In this patient diagnosis was made with only a moderately dense 244K array, whereas the older 2.6K bacterial artificial chromosome array failed to detect this 3-Mb duplication.

Genetic testing for developmental delay: keep searching for an answer

Construction and characterization of a bacterial artificial chromosome (BAC) library of hexaploid wheat (Triticum aestivum L.) and validation of genome coverage using locus-specific primers.

Construction and characterization of a BAC library of a cold-tolerant hexaploid wheat cultivar

Abbreviations: BAC, bacterial artificial chromosome; MAS, marker-assisted selection; SNP, single nucleotide polymorphism.

Marker-assisted selection in public breeding programs: the wheat experience

Bacterial artificial chromosome libraries have also been used to develop physical maps for genomic regions containing resistance gene analog sequences (Marek and Shoemaker, 1997).

Construction and characterization of a deep-coverage bacterial artificial chromosome library for maize. (Cell Biology & Molecular Genetics)

Three bacterial artificial chromosomes (BAC-E7, BAC-22, BAC-C8) located in a breakpoint fragment from AF4 (on chromosome 4) were investigated in a similar manner.

Is topoisomerase II implicated in translocations involving the MLL gene?

The main tools of GENSAT are bacterial artificial chromosomes (BACs), which are simple loops of bacterial DNA that reproduce outside the cell.

BAC to the future

Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes. Proc.

Differences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic species of Crassostrea (Bivalvia, Mollusca)

Delitto Perfetto could also be used to modify large bacterial artificial chromosomes using recently developed strains of Escherichia coli that have proven efficient for site-specific targeting.

Delitto Perfetto: foreign DNA disappears without a trace. (Innovations)

Metaphase and interphase fluorescence in situ hybridization mapping of rice genome with bacterial artificial chromosomes. Proc.

C-Banding and localization of 18S-5.8S-26S rDNA in tall oatgrass species. (Crop Breeding, Genetics & Cytology)

Identification of bacterial artificial chromosomes (BAC) carrying the most closely linked markers is an important step toward cloning of the gene.

Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice, O. sativa. (Cell Biology & Molecular Genetics)

The process involves cutting DNA into fragments of about 200,000 base pairs called bacterial artificial chromosomes (BACs), taking note to record the position of the BACs on the genome, cloning the BACs in bacteria (such as Escherichia coli), determining the sequence of the base pairs, and then reassembling the BACs in their original order using a computer.

Charting the Map of Life

These papers include the development of physical maps using yeast artificial chromosome (YAC) clones and bacterial artificial chromosomes (BACs) and the construction of a rice contig map through a combination of fingerprinting and hybridization.

Oryza: from Molecule to Plant