The structure of the heterotetramer complex of the Wzm permease and the N-terminal ATPase domain of Wzt from Aquifex aeolicus and the structure of the C-terminal carbohydrate-binding domain of Wzt from E.
Munoz et al., "WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis," Journal of Biological Chemistry, vol.
Archaoglobus fulgidus 2.18 2437 Methanobacterium thermotautotrophicum 1.75 1855 Methanococcusjannaschii 1.66 1749 Pyrococcus horikoshii 1.74 2061
Aquifex aeolicus 1.50 1521 Bacillus subtilis 4.20 4100 Borrelia burgdorferi 1.44 1751 Chlamydia pneumoniae 1.23 1073 C.
Aquifex aeolicus (bacteria that grows at 85 [degrees] to 95 [degrees] C!)
The autotrophic bacterium Aquifex aeolicus had been completely sequenced, was partially annotated, lay near the root of the ribosomal phylogenetic tree (Deckert et al., 1998), and was available on the KEGG database (KEGG Pathway Database, 1995).
Reviewing the list of autotrophic extremophiles whose genomes have been completely sequenced and are available in the public domain, we selected Aquifex aeolicus as the central candidate for developing a complete chart of anabolic intermediary metabolism.