Membrane were incubated with primary antibodies anti-phospho-Akt (Ser473) (1:500 dilution, Cell Signaling Technology), anti-
Akt (1:1000 dilution), anti-pAkt (1:1000 dilution), and anti-tubulin (1:2000 dilution).
Formononetin inhibits migration and invasion of mda-mb-231 and 4t1 breast cancer cells by suppressing mmp-2 and mmp-9 through pi3k/
akt signaling pathways.
It also activates
Akt, thereby enhancing mTORC1 functions.
An EGFR and
AKT Signaling Pathway was Identified with Mediation Model in Osteosarcomas Clinical Study.
AKT has more recently been identified as a key player in promoting "synaptic plasticity," the brain's ability to strengthen cellular connections in response to experience.
Recent studies displayed that
AKT signaling and PPAR[gamma] have a mutual inhibitory effect [25-27], but this theory is still controversial.
Inactive
AKT binds PIP3 which enables 3-phosphoinositide-dependent kinase-1 (PDK1) to phosphorylate
AKT at Thr308.
Changes of
Akt, p-Akt, GSK-3[beta], and p-GSK-3[beta] Protein Levels in the Myocardial Tissue.
SC79 (Abcam, ab146428) was used as an
AKT activator.
A rabbit polyclonal antibody against rat
Akt (1 : 300, Abcam, USA), a rabbit monoclonal antibody against rat NF-[kappa]B p50 (1:100, Abcam, USA), a rabbit polyclonal antibody against rat Bax (1:50, Abcam, USA), and a rabbit polyclonal antibody against rat Bcl-2 (1 : 100, Cell Signaling Technology, USA) were used as primary antibodies.
Also termed RAC-PK ("related to the A and C kinases"), the
Akt isoforms comprise a family of three members -Akt1 (PKB-[alpha]), Akt2 (PKB-[beta]), and Akt3 (PKB[gamma])--and their activities are very susceptible to metabolic alterations such as obesity, insulin resistance, and type 2 diabetes (18).