Touch thresholds at the lateral malleolus were measured with a Semmes-Weinstein Pressure
Aesthesiometer [23].
Tactile hypersensitivity was measured in the rats subjected to CCI 12 days after surgery by the use of an automatic von Frey apparatus (Dynamic Plantar
Aesthesiometer, Ugo Basile, Italy).
The Schirmer tear test (STT) (Schirmer test--Ophthalmos, Sao Paulo, Brazil), breakup time test (BUTT) (Fluoresceina strips--Ophtalmos, Sao Paulo, Brazil) and Cochet-Bonnet aesthesiometry (C-B) (Cochet-Bonnet
aesthesiometer, Luneau Ophthalmologie, Paris), starting with 4-cm stimulus length, were conducted under physical restraint.
Touch thresholds were measured with a Semmes-Weinstein Pressure
Aesthesiometer [29].
An UGO-Basile Dynamic Plantar
Aesthesiometer (UGO 37450, Milan, Italy), with an improved calibrated von Frey filaments method [21, 24, 25], was operated by a trained investigator who was blind to the experimental allocation.
Three measures of peripheral sensation were made: tactile sensitivity at the ankle (assessed using a Semmes - Weinstein Pressure
Aesthesiometer) [14], vibration sense at the knee (assessed using an electronic device which drove a 20O Hz vibration of varying intensity), and proprioception, which was tested using an apparatus which measured any error in matching the position of the lower limbs (measured in degrees) [12].