radioimmunoassay /ra·dio·im·mu·no·as·say/ (-im″u-no-as´a) a highly sensitive and specific assay method that uses the competition between radiolabeled and unlabeled substances in an antigen-antibody reaction to determine the concentration of the unlabeled substance, which may be an antibody or a substance against which specific antibodies can be produced.
radioimmunoassay (RIA)
radioimmunoassay (RIA) [ra″de-o-im″u-no-as´a]
radioimmunoassay
ra·di·o·im·mu·no·as·say (r  d - - my -n- s , --my -)n. Abbr. RIA The immunoassay of a substance, such as a hormone or an enzyme that has been radiolabeled. |
radioimmunoassay (RIA)
[rā′dē·ō·im′yənō·as′ā]
Etymology: L, radius + immunis, free; Fr, essayer, to try
a technique in radiology used to determine the concentration of an antigen, antibody, or other protein in the serum. The technique involves the injection of a known amount of a radioactively labeled substance that reacts with the protein in question.
radioimmunoassay (RIA) [ra″de-o-im″u-no-as´a]
a sensitive assay method that can be used for the measurement of minute quantities of specific antibodies or any antigen, such as a hormone or drug, against which specific antibodies can be raised. An assay for a specific hormone uses antihormone antibody produced by injecting the human hormone into an animal, such as a rabbit, and hormone that has been labeled with a radioisotope. These are mixed with the assay specimen and the antigen (hormone) bound to antibody is separated from the unbound antigen by chromatography or other means. Because any hormone in the assay specimen competes with the radiolabeled hormone for antibody binding sites, the amount of hormone in the specimen is inversely proportional to the radioactivity of the bound fraction or directly proportional to the activity of the free fraction. This is the standard method for clinical laboratory measurements of hormones and is also used for therapeutic drug monitoring, drug abuse screening, and other laboratory tests.
radioimmunoassay
a sensitive assay method used for the measurement of minute quantities of specific antibodies or any antigen, such as hormones or drugs, against which specific antibodies can be raised; abbreviated RIA. An assay for a specific hormone uses antihormone antibody produced by injecting the human hormone into an animal, such as a rabbit. In the assay either the antigen or antibody is labeled with radioisotope.
radioimmunoassay
see RIA
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