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Shotgun Sequencing
(redirected from Whole-genome shotgun)

   Also found in: Wikipedia 0.01 sec.
Shotgun Sequencing
The sequencing of a large DNA segment by sequencing of randomly-derived subsegments, whose order and orientation within the large segment are unknown until the final assembly of overlapping sequences.


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As indicated by its name, the GS FLX is an extremely flexible system supporting all sequencing applications, ranging from whole-genome shotgun sequencing to cDNA transcriptome sequencing, to ultradeep sequencing of targeted gene amplicons.
The genome of Aspergillus oryzae was determined by the whole-genome shotgun sequencing method and verified by the optical mapping method.
All three groups used the whole-genome shotgun sequencing method, which decodes multiple short fragments of DNA.
 
 
 
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