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Whole Genome Shotgun Sequencing

   Also found in: Wikipedia 0.01 sec.
Whole Genome Shotgun Sequencing
A semi-automated sequencing technique for determining the entire sequence of a particular organism, in which high-molecular-weight DNA is sheared into random fragments, size selected—usually 2, 10, 50, and 150 kb—and cloned into an appropriate vector


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In conjunction with the significant preliminary mapping and sequencing progress contributed by Jean Weissenbach's group at GenoScope in Envry, France, Agencourt provided another 2 million double-ended whole genome shotgun sequencing reads required to unveil the mystery of this genome.
Myers, a group leader at Janelia Farm, joins Helicos' advisory board with extensive experience in genomic bioinformatics, including the initial proposal and eventual accomplishment of the whole genome shotgun sequencing of the Drosophila, Human, Mouse, and Anopheles genomes.
With genome centers' increasing reliance on whole genome shotgun sequencing, fosmid end sequencing has become a necessity for every genome project.
 
 
 
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