Western blotting


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Western blotting

A method of detecting very small quantities of a protein of interest in a cell or body fluid. The sample is spread by ELECTROPHORESIS on a block of polyacrylamide gel. The separated proteins are then transferred, by blotting, to a thin plastic sheet to make them more accessible for reaction with an antibody specific for the protein of interest. The antibody-antigen complex can then be detected by rinsing the sheet with a second, radioactive, antibody that recognises the first. The sheet will now produce a dark band on X-ray film. See also SOUTHERN BLOTTING.

Western transfer

or

Western blotting

a method for transferring proteins, that have been separated by GEL ELECTROPHORESIS, from the gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
Western blot
Western blot analysis,
, Western blotting (wes'tĕrn blot, blot'ing)
Synonym(s): Western blot analysis.
References in periodicals archive ?
Western blotting consumables including reagents and kits used for testing in different applications
Assessing p70S6 kinase phosphorylation by phospho-flow cytometry is comparable to the Western blotting method
Although a more elegant solution, multiplex fluorescent western blotting requires optimization of blocking reagents, antibody concentrations and incubation times, while users need to be mindful of challenges like antibody cross-reactivity.
The value of the insights provided by Western blotting has kept it relevant for more than 30 years, but its shortcomings are well-recognized.
1% Tween 20, and the antibodies were detected with horseradish peroxidase-conjugated secondary IgG using an Enhanced Chemiluminescence (ECL) Western Blotting Detection Kit according to the manufacturer's instructions (Amersham Pharmacia Biotech).
With manufacturing facilities in Pennsylvania, Rockland produces antibodies for western blotting, immunohistochemistry (IHC), immunofluorescence microscopy, ELISA and FLOW cytometry.
The subject of the contract is the delivery, installation and commissioning of a new, previously unused equipment for electrophoresis and western blotting elektrofokusaci.
ProteinSimple offers high-throughput Western blotting instruments which boosts lab productivity.
Thus, Western blotting and cross-absorption strongly supported that the infection was due to R.
a global leader focused on the health and safety of people and the environment, today announced the launch of Western BLAST[TM], an amplification system used to enhance and simplify Western Blotting.
Equipment for PAGE and Western blotting and for the determination of nucleic acids and