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Western blottingA method of detecting very small quantities of a protein of interest in a cell or body fluid. The sample is spread by ELECTROPHORESIS on a block of polyacrylamide gel. The separated proteins are then transferred, by blotting, to a thin plastic sheet to make them more accessible for reaction with an antibody specific for the protein of interest. The antibody-antigen complex can then be detected by rinsing the sheet with a second, radioactive, antibody that recognises the first. The sheet will now produce a dark band on X-ray film. See also SOUTHERN BLOTTING.
Western blottinga method for transferring proteins, that have been separated by GEL ELECTROPHORESIS, from the gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
Western blot analysis,, Western blotting (wes'tĕrn blot, blot'ing)
Synonym(s): Western blot analysis.