Western blotting


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Western blotting

A method of detecting very small quantities of a protein of interest in a cell or body fluid. The sample is spread by ELECTROPHORESIS on a block of polyacrylamide gel. The separated proteins are then transferred, by blotting, to a thin plastic sheet to make them more accessible for reaction with an antibody specific for the protein of interest. The antibody-antigen complex can then be detected by rinsing the sheet with a second, radioactive, antibody that recognises the first. The sheet will now produce a dark band on X-ray film. See also SOUTHERN BLOTTING.

Western transfer

or

Western blotting

a method for transferring proteins, that have been separated by GEL ELECTROPHORESIS, from the gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
Western blot
Western blot analysis,
, Western blotting (wes'tĕrn blot, blot'ing)
Synonym(s): Western blot analysis.
References in periodicals archive ?
On the basis of applications, the Western Blotting Market is segmented into disease diagnosis, agriculture, biomedical and biochemical research, and others (QC test in biopharmaceutical industry and biologics).
The subject of the contract is the delivery, installation and commissioning of a new, previously unused equipment for electrophoresis and western blotting elektrofokusaci.
1% Tween 20, and the antibodies were detected with horseradish peroxidase-conjugated secondary IgG using an Enhanced Chemiluminescence (ECL) Western Blotting Detection Kit according to the manufacturer's instructions (Amersham Pharmacia Biotech).
Rockland's GFP western blot kit has been validated for use in Western Blotting.
Western blotting is widely used life science analytical technique for detecting proteins in tissue homogenate or extract.
11 /PRNewswire/ -- LI-COR Biosciences announces an updated Infrared Fluorescent Products Guide offering accessories and reagent products including infrared-labeled secondary antibodies, protein labeling kits, Western blotting kits, In-Cell Western(TM) assay kits, Western blotting buffers, and protein stains.
a global leader focused on the health and safety of people and the environment, today announced the launch of Western BLAST[TM], an amplification system used to enhance and simplify Western Blotting.
Equipment for PAGE and Western blotting and for the determination of nucleic acids and
Geschwender says that in-cell detection is faster and easier than traditional Western blotting, and avoids artifacts caused by working with cell lysates.
Thus, Western blotting and cross-absorption strongly supported that the infection was due to R.
The LAS-3000mini is a compact image analysis system designed for chemiluminescence and bioluminescence analysis, specifically for Western blotting applications.