Western blot analysis


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Related to Western blot analysis: immunoblot

West·ern blot a·nal·y·sis

a procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pretagged or posttagged with a labeled secondary protein.
See also: immunoblot.
[coined to distinguish it from eponymic Southern blot a.]

Western blot analysis

n.
A technique for identifying specific antibodies or proteins in which proteins are separated by electrophoresis, transferred to nitrocellulose, and reacted with antibody.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or posttagged with a labeled secondary protein.
Synonym(s): Western blot, Western blotting.
References in periodicals archive ?
b) Western blot analysis of PAI-1 in LA-4 cells which were transfected with 20 or 40 nM siRNA-PAI-1 or with siRNA-Con for 24 hours.
Western blot analysis for caspase-3 in whole ovarian tissues showed that caspase-3 activation was significantly increased by BPA exposure (Figure 2A) in a dose-dependent manner (Figure 2B).
Western blot analysis showed that primary antibody strongly bound to growth hormone (Figure 3).
To study the protein expression western blot analysis of plastidic proteins was done in seedlings grown in dark or above mentioned light regimes.
Diagnostic potential of western blot analysis of sera from dogs with leihsmaniasis in endemic areas and significance of the pattern.
For Western blot analysis, the same procedure was taken as described for anti- His antibody with minor differences.
The active cathelicidin protein was then detected in sebaceous glands using the anti-LL-37 immunoglobulin and by western blot analysis.
For Western blot analysis (n = 5), peptide fractions obtained from affinity chromatography were incubated overnight at 37 [degrees]C with 0.
Detection of the N-Methyl-D-Aspartate Receptor Subunit-1 (NMDAR-1) in the Hypothalamus of the Sheep by Western Blot Analysis.
ZAP-70 expression was assessed by FC in 45 consecutive newly diagnosed CLL patients, and the results were correlated with those of immunocytochemistry and Western blot analysis.
The expression of the integrated aroA-M1 gene was confirmed by western blot analysis of eight individual transformed plants with antiserum raised against EPSPS.