Western blot


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An assay that detects specific proteins within a protein mixture by a multistep process consisting of electrophoresis on a slab gel, transfer of the proteins on the gel to a membrane followed by identification of the specific proteins by antibody staining

Western blot

Immunoblot Molecular diagnostics An assay that IDs antibodies to proteins of specific molecular weights, used to confirm HIV infection–after a positive ELISA screening assay, Lyme disease. See Blot, Electrophoresis, Protein. Cf Northern blot, Southern blot.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]

Western blot

A technique developed in 1979 that is used to confirm ELISA results. HIV antigen is purified by electrophoresis and attached by blotting to a nylon or nitrocellulose filter. The patient's serum is reacted against the filter, followed by treatment with developing chemicals that allow HIV antibody to show up as a colored patch or blot. If the patient is HIV-positive, there will be stripes at specific locations for two or more viral proteins. A negative result is blank.
Mentioned in: AIDS Tests

Western blot,

n.pr a confirmatory test for HIV exposure that identifies antibodies to HIV proteins and glycoproteins.

Western blot

an immunologic technique for the detection of proteins using a radioactive probe. Called also Western transfer.
References in periodicals archive ?
The aim is to replace the currently used multi-step Western blot analysis with the appropriate instrumental method, which does not require manipulation of gels, the use of different device sequences and manually demanding technological procedures.
The bioexpression of PKC[delta] from the cytoplasm to membrane was determined by Western blot analysis.
X-ray film remains the most widely accepted and commonly used detection technique for visualizing chemiluminescent blots, which are the most popular type of western blot.
Indirect immunofluroescence assay (IFA) is also FDA-approved for confirming HIV-I infection, but because the Western blot is used by more than 98% of laboratories in the U.
7) Western blot was positive in 50% of samples identified as equivocal by ECLIA, which is also in agreement with the results of a previous study.
The Cambridge kit is one of two HIV-1 Western blot (WB) kits licensed by the Food and Drug Administration (FDA) for supplemental testing of serum, plasma, and dried whole-blood spot specimens obtained for medical diagnosis or blood and plasma donor screening.
Repeat HIV serologic testing showed a positive HIV enzyme immunoassay and a positive Western blot with bands at gp120, gp160, and p24.
The Western blot is somewhat similar, but uses an electrical field that separates out the various components by their molecular weight.
Thus, if a Western blot comes up negative and the new test comes up positive, it can be difficult to know whether the former is a false negative or the latter a false positive.
This is where western blot technology excels as a common follow up diagnostic test, offering a 100% accurate result.
The FluorChem Q is a CCD-based imaging system that rivals laser-based scanners for quantitative Western blot imaging.
Line blot and western blot immunoassay for diagnosis of Mediterranean spotted fever.