Taq polymerase


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Taq polymerase

 [tak]
an enzyme found in the bacillus Thermus aquaticus, which lives in hot springs; it is heat resistant and thus can endure the high temperatures of the polymerase chain reaction.

Taq pol·ym·er·ase

a temperature-resistant DNA polymerase isolated from Thermus aquaticus that can extend primers at high temperatures; used in the polymerase chain reaction.

Taq polymerase

[tak]
an enzyme found in the bacillus Thermus aquaticus, which lives in hot springs. It is heat resistant and thus can endure the high temperatures of the polymerase chain reaction.

polymerase

an enzyme that catalyzes polymerization, particularly of nucleic acids.

polymerase chain reaction
see pcr1.
RNA polymerase
1. an enzyme that synthesizes an RNA copy of the sequence in a limited region of DNA in a process known as DNA transcription. Called also DNA-dependent RNA polymerase.
2. a viral enzyme that synthesizes RNA from an RNA template during viral replication. Called also RNA-dependent RNA polymerase.
RNA-dependent RNA polymerase
see RNA polymerase (above).
Taq polymerase
a DNA polymerase that functions at high temperature; derived from the bacterium Thermus aquaticus and used in the polymerase chain reaction.
References in periodicals archive ?
We evaluated the use of a supplemented PCR reagent mixture with more Taq polymerase, Mg[Cl.
In order to compensate for the chelating effect on magnesium, we performed some PCRs with DNA samples obtained through the use of increasing concentrations (up to 4 mM) of magnesium chloride, bearing in mind that magnesium concentrations higher than 4 mM inhibit Taq polymerase.
Three micrograms of cDNA were added to the respective oligonucleotide primers (1 nM each) and Taq polymerase (2.
Madison, WI 12/8/99-A US federal district court has ruled that the '818 patent for Taq polymerase, owned by Hoffman-LaRoche was obtained by fraud and thus is unenforceable.
PCR techniques have long been used since 1985 to amplify short strands of DNA, and since the expiry of the Taq polymerase patent in 2007, the market has been growing quickly.
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2] between 4 and 5 mmol/L produced the fastest extension rates for Titanium and Klentaq I, 2 deletion variants of Taq polymerase, with decreasing rates at higher concentrations.
Following set of reverse and forward primers was used in the PCR amplification of Mycobacterium DNA: TB-1 5' gtg cgg atg gtc gca gag at 3' TB-2 5' ctc gtc cag cgc cgc ttc g 3' TB-3 5' ctc gat gcc ctc acg gtt ca 3' TB-4 5' cct gcg agc gta ggc gtc gg 3' The PCR reaction mixture (20uL) contained 1mM dNTPs, 2mM MgCl2, 1 ul each of 40 pM of each of the forward and reverse primers, 1X Taq buffer, 1U of Taq polymerase.
see Figure 1) utilizes the 5' exonuclease activity of Taq polymerase as originally described in 1991 by Holland et al.
In order to improve the PCR sensitivity, the blood samples were processed rapidly, within 48 hours of collection, to prevent Taq polymerase inhibition.