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The tumor cells were positive for TTF-1 (Figure 2, B) and napsin A (Figure 2, C), but negative for p40 (Figure 2, D) and CK5/6 (Figure 2, E).
Immunohistochemical studies were carried out on the cell block and core biopsy sections using antibodies TTF-1 (1:200; Leica), thyroglobulin (1:1000; Dako), ER (1:50; Dako), PR (premade; Leica), GATA3 (1:200; Cell Marque), GCDFP-15 (1:500; Leica), CD56 (Premade; Leica), synaptophysin (1:200; Dako), chromogranin (1:200; Cell Marque), pAx8 (1:100; Cell Marque), villin (1:100; Leica), CK 7 (1:400; Dako) and CK20 (1:200; Novocastra).
The surface cells were positive for TTF-1, EMA, and cytokeratin.
2] In this case, the SRS was negative and the primary site could have been missed if a CT scan of the thorax and additional histological investigations, like a TTF-1, would not have been performed.
These, in combination with some of the more traditional markers such as TTF-1, CK5 and p63, may enable much more confident identification of SqCC and LADC.
The tumor was focally positive for CD68 (Table 2) and negative for CD45, CD1a, PAX5, CD34, CD117, CD15, CD23, CD4, HMB45, CK20, CK7, synaptophysin, chromogranin, CDX-2, PSA, PSAP, and TTF-1 (Table 3).
A reacao para o TTF-1 foi nuclear e observada focalmente, principalmente nas margens das massas tumorais (Figura 1E).
Bulgular: En sik kullanilan isaretleyiciler epiteloid malign mezotelyoma icin kalretinin ve CK5/6, adenokarsinom icin CEA, CD15 ve TTF-1 idi.
For reclassifying tumors with an original diagnosis of large cell carcinoma or NSCLC, not otherwise specified, and for determining thyroid transcription factor 1 (TTF-1) and p40 expression, recut sections 4 [micro]m thick were obtained from tissue microarray blocks and stained with 2 dual-antibody cocktails for DG3 + CK5/napsin A and p40/TTF-1 (prediluted; napsin A and p40, rabbit polyclonal; DG3 + CK5 and TTF-1, mouse monoclonal; Biocare Medical) as previously described.
It can be permanently mounted in a variety of mounting media and is suitable for both Iimmunohis-tochemistry and in-situ hybridization applications including HPV, CMV, EBV, p63, TTF-1, Ki-67, and other target antigens such as blood and lymphatic vessels, and basal and myoepithelial cells.
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