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DNA isolated from both fresh samples and formalin-fixed, paraffin-embedded tissues had an identical migration pattern on the TGGE analysis.
The TGGE analysis of liver, bone marrow biopsy, and peritoneal fluid all showed an identical banding pattern.
Detection of a predominant monoclonal DNA fragment by TGGE relies on the same principle on which DGGE is based.
The different migration pattern of PCR products detected by TGGE is particularly important for routine diagnostic analysis, since PCR contamination may cause false positivity.
Scheller et al[23] used TCR[Gamma] primers for PCR amplification and compared heteroduplex analysis, sequencing gel analysis, and TGGE for detection of amplification products.
4] Nonstandard abbreviafions: apo B, apolipoprotein B; LDL-C, LDL-cholesterol; FDB, familial defecfive apo B-100; CAD, coronary artery disease; TGGE, temperature gradient gel electrophoresis; HLP, hyperlipoproteinemia; HDL-C, HDL-cholesterol; RFLP, restricfion fragment length polymorphism; and SSCP, single-strand conformation polymorphism.
Positions and sequences of primers used for TGGE PCRs and RFLP typing.
Detection limit Maximum (minimal ratio of fragment size Detectable mutant to wild-type Method (kb) mutations cells) DGGE, TGGE 1 Close to ?