Central area of the cystic spaces contain granular, basophilic, filamentous material surrounded by Splendore-Hoeppli Phenomenon along with neutrophilic microabscesses suggestive of botryomycosis [Fig.
6,7] This typical histologic appearance is referred to as the Splendore-Hoeppli phenomenon and can be seen using gram stain, Giemsa stain, PAS stain or silver stain.
H&E is a versatile stain that enables the pathologist to evaluate the host response, including the Splendore-Hoeppli phenomenon
, and to detect other microorganisms.
Fungal hyphae associated with a Splendore-Hoeppli phenomenon were visible in the left leg muscle.
Grocott staining revealed numerous septate hyphae in these same areas of muscle; some fungal hyphae were surrounded by intensely eosinophilic material in asteroid- or star-like configurations characteristic of the Splendore-Hoeppli phenomenon.
Zygomycosis can be distinguished from infections caused by mucoraceous fungi by a lack of vascular invasion or infarction and the production of a prolific chronic inflammatory response and Splendore-Hoeppli phenomenon around the hyphae.
Splendore-Hoeppli phenomenon was observed on microscopic examination.
Histopathologic examination showed extensive inflammation, granulomas with eosinophils and numerous fungal hyphae, and zygospores surrounded by eosinophilic material called the Splendore-Hoeppli phenomenon.
Most reported cases of basidiobolomycosis show the presence of a prominent or intense Splendore-Hoeppli phenomenon surrounding fungal elements.
Histopathologic evaluation of the mass revealed marked inflammation and the Splendore-Hoeppli phenomenon
, in which eosinophils are deposited around the fungus.
is found in association with such infections as (Figure 2): [FIGURE 2 OMITTED] a.
The Splendore-Hoeppli phenomenon
describes the eosinophilic, pseudomycotic structures composed of necrotic debris and immunoglobulin.