Southern transfer


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Southern transfer

or

Southern blotting

a method of transferring BANDS of DNA, separated by GEL ELECTROPHORESIS, to a membrane support. The technique is named after E. Southern, who devised it. Compare NORTHERN TRANSFER.

Southern transfer

a method for performing nucleic acid hybridizations in which fragments of DNA separated usually in agarose gels are transferred (blotted) from the gel to nitrocellulose or nylon membranes. The transferred fragments are then probed with a radiolabeled fragment of DNA. Called also Southern blot.
References in periodicals archive ?
Derry are on the verge of sealing a deal which could see the 22-year-old join the Candystripes in January when the southern transfer window re-opens, with Hamilton keen to loan the player out in the interim period.
The book covers all of the relevant topics: introductory genetics, DNA-based technologies such as the Southern transfer and PCR, human genetic variations, bioinformatics, proteomics, and the ethical issues that will likely play more of a key role in the adaptation of genomic information to clinical practice than will the technology itself.
Expensive optional equipment performs automated Southern transfer, automated DNA extraction, and image analysis.
Geiger-Mueller counter Beta-particle counter Beta-particle shielding devices Nucleic acid extractor (optional) Electrophoresis units ([greater than]1) Automated electrophoresis/automated Southern transfer unit (optional) Transfer units (vacuum or capillary) Autoradiography cassettes/intensifying screens Dot/slot blot apparatus Densitometer or image analysis system
Tenders are invited for Contract for Operation Maintenance Repair for Bisalpur Jaipur Water Supply Project Transmission System with Central Western Southern Transfer mains Pumping stations at Balawala Jawahar Circle Mansarover Ramniwas Bagh Central Park Amanishah SCADA Associated work including cost of all material labour consumables chlorine spare part etc for a period of five years except power with separate payables for Major repairs)
The results correlated very well with the results of Southern transfer analysis.
These recombination events or CMT1A duplications were directly detected by Southern transfer analysis [22,26] and the 3.
It Is Approximately 1200 Feet Long Originating At The Southern Transfer Sump, Continuing Through A Valve Manhole At The Corner Of Gleam Street And Terminating At The Leachate Storage Tanks.
Molecular diagnostic assays for the evaluation of lymphoid malignancies including Southern transfer and PCR have become increasingly popular in the clinical laboratory.
DETERMINATION OF B- AND T-CELL CLONALITY BY SOUTHERN TRANSFER ANALYSIS
231) in which the oncogene amplification had been previously measured with Southern transfer analysis (Table 2).

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