Southern blotting


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A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity

Southern blotting

A method of identifying a fragment of DNA containing a specific sequence of bases. A mixture of fragments, produced by cutting DNA with restriction enzymes, is separated on a gel block by ELECTROPHORESIS. The fragments are denatured to single strand DNA and transferred, by blotting, to a nitrocellulose sheet. The position of the desired fragment can then be shown by hybridizing it with a DNA probe labelled with radioactive phosphorous that will cause a black line on an X-ray film. The method was named after its developer Edward M. Southern. A similar technique for RNA analysis has been humorously called ‘Northern blotting’ and the play on words has been extended to include ‘WESTERN BLOTTING’.

Southern transfer

or

Southern blotting

a method of transferring BANDS of DNA, separated by GEL ELECTROPHORESIS, to a membrane support. The technique is named after E. Southern, who devised it. Compare NORTHERN TRANSFER.
References in periodicals archive ?
Southern blotting slightly enhanced the sensitivity of the PCR, but visual inspection of gel-resolved PCR products from feces was nearly equally sufficient (Table and Figure).
A total of 76 kids were generated, among them 5 kids tested positive by Southern blotting analysis.
Predicted restriction enzyme cut sites in the database sequences were compared with actual restriction mapping fragment sizes and with the data from Southern blotting to determine the order of sequence contigs, An independent sequence assembly for this region obtained from Celera Genomics (GA_x6K02T2PU9B, as of 1 June 2002) was also used to fill in some gaps.
The Sequenom EpiTYPER system (which uses MALDI-TOF mass spectrometry for quantification) and Southern blotting tools were used to determine the methylation output ratio and the activation ratio, respectively.
Southern blotting identified a plasmid of [approximately equal to] 150 kb harboring [bla.
The purified DNA can be used in a number of downstream applications including Real-Time PCR, Southern blotting and sequencing.
Step-by-step instructions are provided for staining microbes, viewing meiosis under the microscope, extracting chromatin, culturing peripheral blood lymphocytes, southern blotting, and isolating chloroplasts from spinach leaves.
Although all steps prior to Southern blotting had positive results, no bands were seen on the exposed film: therefore the presence and number of correctly targeted colonies could not be determined.
When the familiar broad smear of restricted DNA is visible after 50-100 volt-hours, full-scale electrophoresis for Southern blotting may commence.
In addition, the study revealed novel patterns of methylation mosaicism associated with female fragile X carriers that cannot be detected by Southern blotting.
In our laboratory, Southern blotting was conducted according to an established protocol (27) to confirm chimeric CYP21A1P/CYP21A2 genes.
After AluI digestion, the DNA was transferred to a nylon membrane for polymorphic guanine cytosine-rich repetitive sequence (PGRS) Southern blotting.

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