Southern blot test

Southern blot test

[suth′ərn]
a gene analysis method used in identification of specific deoxyribonucleic acid (DNA) fragments and in diagnosis of cancers and hemoglobinopathies. It involves the placement of a nitrocellulose film on agarose gel surfaces, with dry blotting material on the film. Liquid is then transported from a reservoir beneath the gel through the gel and nitrocellulose layer. The film adsorbs the DNA fragments. The fragments are then analyzed for rearrangements in immunoglobulin or cell receptor genes, chromosomal translocations, oncogene amplifications, and point mutations within oncogenes. Immunoglobulins and T cell receptor genes bear signatures that identify various leukemias and lymphomas. See also Northern blot test.

Southern Blot test

An analytical method traditionally used in DNA analysis. After a sample of DNA fragments is separated by agarose gel electrophoresis, the fragments are transferred to a solid cellulose support by blotting. The gel is placed between a concentrated salt solution and absorbent paper. Capillary action draws the fragments onto the solid support. The support is then treated with radiolabeled DNA probes.

Southern blot test,

n.pr a gene analysis method used to identify specific deoxyribonucleic acid (DNA) fragments and diagnose cancers and hemoglobinopathies.
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The TP PCR amplification method amplifies within the expansion, enabling rapid identification of large pathogenic CAG repeats that otherwise may only be detected with a follow-up Southern blot test.
Due to the labor and reagent costs involved, large Southern blot test volumes typically do not occur.

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