Southern blot hybridization

Southern blot hybridization

Southern blotting Molecular biology A method delineated by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis. See Autoradiography, Base sequence, Complementary sequence, DNA, Electrophoresis, Probe. Cf Northern blot, Western blot.
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As established by Pfister et al (10) and Ozden et al, (4) HPV subtypes 13 and 32 have been identified as the cause for FEH lesions through polymerase chain reaction (PCR) analysis and Southern blot hybridization, respectively.
RacPyV does not appear to be integrated into the host genome because both Southern blot hybridization and RCA analyses indicate that full-length genome exists in an episomal, circular state.
9) More recently, molecular techniques such as Southern blot hybridization, polymerase chain reaction, and in situ hybridization demonstrated direct evidence for an association between EBV and Hodgkin lymphoma.
Diagnostic sensitivity of polymerase chain reaction and Southern blot hybridization for the detection of human papillomavirus DNA in biopsy specimens from cervical lesions.
Typically, the TAT for Southern blot hybridization is 10-14 days, making such testing impractical, particularly to diagnose acute leukemias and monitor patients.
We used PCR amplification and Southern blot hybridization to detect 9 virulence factors among 40 EHEC type-strains from the STEC Center, National Food Safety and Toxicology Center, Michigan State University (East Lansing, MI, USA).
The topics covered include chromosome analysis; chromosome banding; molecular diagnostic studies, including PCR, allele-specific oligonucleotide hybridization, Southern blot hybridization, and Northern blot hybridization; genetic disorders of metabolism; genetic disorders of plasma proteins; genetic disorders of blood cells; screening for genetic disease; newborn screening; screening for adult-onset genetic disease; carrier detection; and prenatal screening.
1] Human papillomavirus DNA is detectable by using in situ hybridization (ISH), filter ISH, Southern blot hybridization, dot-blot hybridization, and polymerase chain reaction (PCR).
29] The successful extraction of RNA was controlled by the detection of actin mRNA, and the specificity of the amplification product was confirmed by Southern blot hybridization or sequencing.
Note that the sensitivity of PCR testing is enhanced by subjecting the electrophoretic gel that generates data indicative of a neoplastic mutation or the presence of an infecting microorganism's DNA (or RNA) to Southern blot hybridization.
The specificity of the detected noroviruses was confirmed by Southern blot hybridization as described previously (5).

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