Southern blot analysis


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Related to Southern blot analysis: Southern blotting method, Southern transfer, DNA blotting

South·ern blot a·nal·y·sis

(sŭdh'ĕrn),
a procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridized with complementary (labeled) nucleic acid probes.

Southern blot analysis

n.
A technique for identifying specific sequences of DNA in which DNA fragments are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe.

South·ern blot a·nal·y·sis

(sŭdh'ĕrn blot ă-nal'i-sis)
A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridized with complementary (labeled) nucleic acid probes.

Southern,

M.E., 20th century English biologist.
Southern blot analysis - a procedure to separate and identify DNA sequences.
References in periodicals archive ?
Southern blot analysis is considered the gold standard for identifying clonal IGH rearrangements.
Recent improvements in the detection and sizing of large FMR1 expansion mutations by the PCR have reduced the need for Southern blot analysis in fragile X testing (26-28).
2 gene into the orchardgrass genome, transgenic plants were analyzed by genomic DNA PCR and Southern blot analysis.
At the seedling stage, the transgenics were screened by PCR followed by Southern blot analysis for the segregation pattern of the transgenes.
Although Southern blot analysis combined with the PCR is the current gold standard in FXS diagnostics, published data concerning relationships of cognitive status with the FMR1 activation ratio obtained with Southern blotting are inconsistent, perhaps owing to differences in sample selection.
Southern Blot Analysis of the HVA1 Gene in R3 Progeny Plants
Southern blot analysis suggested that a 6-kb EcoRI fragment contained the resistance determinant.
In 1991, Masih et al (11) identified EBV in hyperplastic lymph nodes (16% by dot-blot hybridization, 14% by Southern blot analysis, and 43% by a PCR technique).
Yet molecular characterizations of FMR1 have long relied on Southern blot analysis, particularly to determine the methylation status of the gene.
The AY-glucuronidase activity PCR analysis and high frequency of single gene insert in Southern blot analysis indicated stable transformation of gusA and nptII genes.
For Southern blot analysis, 7-10 [micro]g of isolated DNA was digested with EcoRI and NruI and separated on an 8-g/L agarose gel containing Tris-acetate-EDTA buffer (40 mmol/L Tris-acetate and 1 mmol/L EDTA).
Southern blot analysis typically requires between 2 and 20 ug of DNA for detection of single-copy sequences by radiolabeled molecular probe hybridization: the amount of DNA needed varies by genome size and thus by organism source.

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