Southern blot


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Related to Southern blot: Northern blot
A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity

Southern blot

a technique for detecting specific DNA sequences following agar gel electrophoresis of a set of DNA restriction enzyme digestion fragments. The fragments after electrophoresis are transferred to a nitrocellulose or nylon membrane by applying the membrane to the gel; the membrane is probed with a radioprobe that hybridizes to one or more of the separated fragments and used to produce an autoradiogram. Named for Edward Southern.
References in periodicals archive ?
Arrows, polymerase chain reaction primers to detect homologous recombination; Bar, probe for Southern blot analysis.
Southern Hybridization The presence of a begomovirus was further confirmed by Southern blot hybridization using Cotton leaf curl virus as a general probe for begomoviruses [13].
Los ensayos de Southern blot realizados en este trabajo, usando una sonda construida contra dTdic1, mostraron que la sonda hibridaba sobre el ADN de las muestras, pero no se observaron bandas bien definidas, a pesar de que se evaluaron varias enzimas de restriccion, temperaturas de hibridacion y diferentes concentraciones de ADN (figura 8).
Southern blot analysis by Brevibacterium cholesterol oxidase type II oligo probe.
For Southern blot analysis, 944 results were reported, with a successful response rate of 95%.
Nuclear integration was analyzed by Southern blot hybridization.
En dos familias con hemofilia A y dos con hemofilia B, la mutacion responsable pudo ser detectada por medio de Southern Blot, por la reaccion en cadena de la polimerasa o por secuenciacion genetica.
Figure 2 shows for example the experimental result of a southern blot analysis.
One lysine mutant was characterized as an auxotroph on selective media, and the transposon-inserted gene fragment confirmed as incorporated into the chromosome by a Southern blot (N=1).
Terminal repeat analysis by Southern blot for Epstein-Barr virus was positive.
The presence of sequences complementary to the probe DNA is detected using Southern blot hybridization and autoradiography.
Two of these, a male from Great Barrier Island (GBI) and a female from Toatoa, had also been studied by Southern blot hybridization.

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